Priore - HEALING OF THE ACUTE AND CHRONIC EXPERIMENTAL TRYPANOSOMIASIS BY THE COMBINED ACTION OF MODULATED MAGNETIC ...

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(Cover page)
HEALING OF THE ACUTE AND CHRONIC EXPERIMENTAL TRYPANOSOMIASIS
BY THE COMBINED ACTION
OF MODULATED MAGNETIC FIELDS AND ELECTROMAGNETIC WAVES
A. PRIORE
(Submitted to Bordeaux University, France, in 1974.
Translated from French
(inside page)
To struggle to convince yourself
of the truth that you have glimpsed,
is the first step toward progress ....
To persuade the others is the second ....
There is a third one,
maybe less useful, but very
enviable nonetheless,
which is to convince your adversaries! ....
Louis Pasteur
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(New page)
TABLE OF CONTENT
Pages
INTRODUCTION
1
CHAPTER I: EQUIPMENT AND METHODS
5
1. STUDY OF THE EMITTED RADIATION
5
I. The Plasma
II. The UHF wave
III. The HF Wave
IV. The Magnetic Field
2. THE PARASITES
8
2.1. Trypanosomes
2.1.1. Trypanosoma equiperdum
a. The pathogenic agent
b. Antigenic variation
2.1.2. Trypanosoma gambiense
2.2. Hematozoa
11
3. THE HOST ANIMALS
13
4. THE PARASITIC MODELS
14
4.1. Acute Trypanosomiasis with T. Equiperdum
14
4.1.1. Characteristic of the disease
4.1.2. Antigenic variation
4.2. Chronic trypanosomiasis
16
4.2.1. Experimental Trypanosomiasis of rabbit with T. Equiperdum
a. Evolution of disease
b. Histological study of the testicular lesions
c. Antigenic variation
4.2.2. Experimental Trypanosomiasis of the mouse with T. Gambiense
4.3. Paludism of the mouse with P. Berghei
20
5. METHOD OF STUDY OF HOST ANIMALS EXPERIMENTALLY INFESTED
(IRRADIATED AND NON-IRRADIATED)
21
5.1. Clinical observation
21
5.2. Parasitemia - Negativation
21
5.2.1. Acute Trypanosomiasis of the mouse and of rat with T.
Equiperdum
5.2.2. Chronic Trypanosomiasis of rabbit with T. Equiperdum
5.2.3. Chronic Trypanosomiasis of the mouse with T. gambiense
5.2.4. Paludism of the mouse with P. Berghei
5.3. Study of the immune status
23
5.3.1. Serous levels of G and M immunoglobulins (IgG and IgM)
a. Dosage technique of the G and M immunoglobulins
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b. Evolution of G and M immunoglobulins
during acute trypanosomiasis of the mouse
and rat with T. Equiperdum
c. Evolution of G and M immunoglobulins
during the chronic trypanosomiasis of rabbit
with T. Equiperdum
d. Evolution of the G and M immunoglobulins
during the chronic trypanosomiasis of the
mouse with T. Gambiense.
5.3.2. State of immune protection
5.3.3. Study of certain humoral antibodies
a. (General) antigenic structures of T. Equiperdum
b. Agglutinants antibodies
c. Hemagglutinants antibodies
d. Precipitants antibodies
e. Sero-protective antibodies
f. Evolution of humoral antibodies during the
acute trypanosomiasis of the mouse and rat
with T. Equiperdum
g. Evolution of humoral antibodies during the
chronic trypanosomiasis with T. Equiperdum
5.4. Criteria of parasitological healing
32
5.4.1. Experimental Trypanosomiasis due to T. Equiperdum
5.4.2. Chronic trypanosomiasis of the mouse with T. Gambiense
CHAPTER II: RESULTS
33
1. THE FIRST IRRADIATION EXPERIMENTS
34
1.1. Irradiation experiments with device No 1.
34
HF wavelength of 19 m: experiments A
HF wavelength of 17 m: experiments B
1.2. Irradiation experiments with device No 2: experiments C
34
2. ACUTE TRYPANOSOMIASIS WITH T. EQUIPERDUM: TUNING OF
EXPERIMENTAL CONDITIONS LINKED TO THE PARASITIC MODEL
40
2.1. Duration of the daily irradiation: experiments D
40
2.2. Importance of the inoculum: experiments E
40
2.3. Importance of the time of the start of treatment: experiments F
41
2.4. Influence of preliminary splenectomy: experiments G
42
2.5. Behavior of the dyskinetoplastic strain (Bethesda) of Trypanosoma
equiperdum: Experiments H
42
2.6. Influence of the total duration of treatment: experiments I
(Number of irradiation sessions)
43
2.7. Results obtained in the rat: experiments J
44
2.8. Body temperature of mice treated by the radiation
44
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3. ACUTE TRYPANOSOMIASIS WITH
T. EQUIPERDUM
: TUNING OF
EXPERIMENTAL CONDITIONS LINKED TO RADIATION
46
3.1. Influence of the distance relative to the output face of the plasma tube:
experiments K
46
3.2. Influence of the distance relative to the axis of the plasma tube:
experiments L and M
47
3.3. Synergy of the essential constituents of the radiation: experiments N,
O, P and Q
51
3.4. Influence of the nature of the magnetron: experiments R
52
3.5. Effect of accessory constituents of the radiation: experiments S
52
4. MICE AND RATS INFESTED WITH
T. EQUIPERDUM
: STUDY OF THE
IMMUNE STATUS OBTAINED WITH THE HELP OF TREATMENT BY
IRRADIATION
54
4.1. Serous levels of the IgG and the IgM
54
4.2. Evolution of the humoral antibodies
54
4.3. Study of the state of immune protection - Reinfestation
55
4.3.1. Mice having presented fatal relapses
4.3.2. Mice having evolved toward the healing but after a
parasitemic moderate and temporary relapse.
4.3.3. Mice having evolved directly toward healing: experiments T and U
4.3.4. State of immune protection in the rats infested by T.
Equiperdum and treated by the radiation: experiments
V. Phenomena of therapeutic facilitation: experiment W.
5. CHRONIC TRYPANOSOMIASIS OF RABBIT WITH
T. EQUIPERDUM
EFFECT OF RADIATION
61
5.1. Effect of the treatment by irradiation started 2 hours after infestation:
experiment X
61
5.2. Effect of treatment by irradiation started after a certain time of
evolution of the disease.
63
5.2.1. Evolution of the disease for 2 weeks: experiment Y
5.2.2. Evolution of the diseases for 3 weeks: experiment Z
5.2.3. Evolution of the disease for 4 weeks: experiment AA
5.3. Histological study of the testicular lesions and of their evolution in
rabbits treated by irradiation.
65
5.3.1. Treatment started 2 hours after the infestation
5.3.2. Treatment started after two weeks of evolution of the disease
5.4. Body temperature of rabbits during the treatment
67
5.5. Trypanosomed rabbits healed by the radiation: effect of a reinfestation
not accompanied by irradiation (experiment AB)
68
5.6. Trypanosomed rabbits healed by the radiation: effect of reinfestations
accompanied by irradiations (experiment AC)
68
5.7. Analysis of the sero-protective power: confirmation of its
immunoglobulinic nature (experiment AD)
71
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6. CHRONIC TRYPANOSOMIASIS OF THE MOUSE WITH
T. GAMBIENSE
:
EFFECT OF THE RADIATION (experiments AE and AF)
74
7. PALUDISM OF THE MOUSE WITH
P. BERGHEI
: EFFECT OF RADIATION
(experiments AG)
77
CHAPTER III: DISCUSSION - CONCLUSION
78
1. GENERAL CONSIDERATIONS
78
1.1. Control of inoculated trypanosomes - Control animals
79
1.2. Control Board
79
1.3. Eventual existence of a thermal effect
80
1.4. The effect of the radiation is not always a total effect
80
1.4.1. Value of the HF wavelength
1.4.2. Influence of the power of the UHF wave
1.4.3. The notion of “superior limit of the biological effect of the
radiation”.
1.4.4. Mice infested with T. Equiperdum and placed under
treatment after 48 hours of evolution of the acute
trypanosomiasis
1.4.5. Duration of treatment by irradiation
1.4.6. Effect of the treatment by irradiation on the chronic
trypanosomiasis of the mouse with T. Gambiense
1.4.7. Effect of the radiation in other parasitic models
2. MECHANISM OF OPERATION
84
2.1. Exclusive effect on the trypanosome
84
2.1.1. Propagation of the components of the radiation
throughout the organism
2.1.2. The notion of “superior limit of the biological effect of the
radiation”
2.1.3. The phenomenon of therapeutic facilitation
2.1.4. Irradiation of the trypanosomes maintained alive “in vitro”
2.2. Stimulation of defense mechanisms
86
2.2.1. Evolution of the parasitemia in the mice infested with T.
Equiperdum and treated by irradiation
2.2.2. Decrease of the parasitemia until the negativation
between two seances of irradiation
2.2.3. Influence of the UHF wave power
2.2.4. Effect of the treatment by irradiation on the
trypanosomiasis of the mouse with T. gambiense
2.2.5. Propagation of the components of the radiation in the organism
2.2.6. Phenomenon of therapeutic facilitation
2.2.7. Mice infested with T. Equiperdum and treated after 48
hours of evolution of the disease
2.2.8. Effect of the radiation in other parasitic models.
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